Skip to main content
Addgene

pSY14-ptb
(Plasmid #90490)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSY8
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sceC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSY8 derivative with sceC gene under the control of ptb promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSY14-ptb was a gift from Sheng Yang (Addgene plasmid # 90490 ; http://n2t.net/addgene:90490 ; RRID:Addgene_90490)

  • For your References section:

    I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462
Related items:
Commonly requested with: