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Addgene

pSX6
(Plasmid #90468)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90468 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSE358
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    snR6
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    snR6 (a.k.a. snR6)
  • Promoter native

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSX6 was a gift from Christine Guthrie (Addgene plasmid # 90468 ; http://n2t.net/addgene:90468 ; RRID:Addgene_90468)
  • For your References section:

    Multiple roles for U6 snRNA in the splicing pathway. Madhani HD, Bordonne R, Guthrie C. Genes Dev. 1990 Dec;4(12B):2264-77. PubMed 2149118