pSX6
(Plasmid
#90468)
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PurposeU6 wt in pSE358 (TRP1 CEN)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSE358
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesnR6
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenesnR6 (a.k.a. snR6)
- Promoter native
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSX6 was a gift from Christine Guthrie (Addgene plasmid # 90468 ; http://n2t.net/addgene:90468 ; RRID:Addgene_90468) -
For your References section:
Multiple roles for U6 snRNA in the splicing pathway. Madhani HD, Bordonne R, Guthrie C. Genes Dev. 1990 Dec;4(12B):2264-77. PubMed 2149118