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LARG mCherry sspB in pcDNA3.1
(Plasmid #90459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7687
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LARG DH-PH (aa 766–1138)
  • Alt name
    Rho guanine nucleotide exchange factor 12 (ARHGEF12)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1119
  • GenBank ID
    XM_017017422.1
  • Entrez Gene
    ARHGEF12 (a.k.a. LARG, PRO2792)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    710
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site BspE1 (destroyed during cloning)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    sspB
  • Species
    bacterial
  • Insert Size (bp)
    342
  • Mutation
    R73Q

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspE1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LARG sequence contains an Y830F variant that does not affect plasmid function. The sspB sequence was obtained from pLL7.0: mTiam1(64-437) tgRFPt-SSPBR73Q (Plasmid #60418).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARG mCherry sspB in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 90459 ; http://n2t.net/addgene:90459 ; RRID:Addgene_90459)

  • For your References section:

    Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. O'Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 10.1016/j.devcel.2018.05.029. 10.1016/j.devcel.2018.05.029 PubMed 29937389
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