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Addgene

pMIG
(Plasmid #9044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 9044 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    modified pMSCV 2.2
  • Backbone size (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    MSCV IRES GFP
  • Tag / Fusion Protein
    • IRES GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281.

Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.

Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG was a gift from William Hahn (Addgene plasmid # 9044)
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