-
PurposeA FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA 3
- Total vector size (bp) 5420
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLck10+ Venus+mcherry+ strech of positively charged amino acids
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MCS+ was a gift from Katharina Gaus (Addgene plasmid # 90412 ; http://n2t.net/addgene:90412 ; RRID:Addgene_90412) -
For your References section:
A FRET sensor enables quantitative measurements of membrane charges in live cells. Ma Y, Yamamoto Y, Nicovich PR, Goyette J, Rossy J, Gooding JJ, Gaus K. Nat Biotechnol. 2017 Mar 13. doi: 10.1038/nbt.3828. 10.1038/nbt.3828 PubMed 28288102
Map uploaded by the depositor.
