MCS Lck only
(Plasmid
#90411)
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PurposeA control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA 3
- Total vector size (bp) 5420
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLck10+ Venus+mcherry
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCS Lck only was a gift from Katharina Gaus (Addgene plasmid # 90411 ; http://n2t.net/addgene:90411 ; RRID:Addgene_90411) -
For your References section:
A FRET sensor enables quantitative measurements of membrane charges in live cells. Ma Y, Yamamoto Y, Nicovich PR, Goyette J, Rossy J, Gooding JJ, Gaus K. Nat Biotechnol. 2017 Mar 13. doi: 10.1038/nbt.3828. 10.1038/nbt.3828 PubMed 28288102
Map uploaded by the depositor.
