1201 pAdTrack FKHR H215R AAA
(Plasmid
#9039)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 9039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAdTrack CMV
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 9010
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Vector typeMammalian Expression, Adenoviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKHR H215R AAA
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Alt nameFKHR
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Alt nameFOXO1A
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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MutationH215R T24A S256A S319A
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Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
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Tag
/ Fusion Protein
- flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypcDNA flag FKHR was a gift from Eric Tang.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Inserts liberated by partial HindIII and complete XbaI digestion of pCDNA3-Flag-FKHR were ligated to HindIII/XbaI-restricted pAD-TrackCMV to give pAD-Track-Flag-FKHR. Must recombine into pAdEasy before generating Adenoviral particles.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1201 pAdTrack FKHR H215R AAA was a gift from William Sellers (Addgene plasmid # 9039 ; http://n2t.net/addgene:9039 ; RRID:Addgene_9039) -
For your References section:
A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy S, Nakamura N, Sansal I, Bergeron L, Sellers WR. Cancer Cell. 2002 Jul . 2(1):81-91. 10.1016/S1535-6108(02)00086-7 PubMed 12150827
Map uploaded by the depositor.