1200 pAdTrack FKHR H215R
(Plasmid #9038)

• Depositing Lab: William Sellers
• Publication: Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Backbone

• Vector backbone: pAdTrack CMV
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 9010
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKHR H215R
• Alt name: FKHR
• Alt name: FOXO1A
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: H215R
• Entrez Gene: FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pcDNA flag FKHR was a gift from Eric Tang.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Inserts liberated by partial HindIII and complete XbaI digestion of pCDNA3-Flag-FKHR were ligated to HindIII/XbaI-restricted pAD-TrackCMV to give pAD-Track-Flag-FKHR. Must recombine into pAdEasy before generating Adenoviral particles.