pLKO5d.SFFV.dTomato-miR30n
(Plasmid #90334)

• Purpose: miR30 based shMir knockdown
• Depositing Lab: Dirk Heckl
• Publication: Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10.1038/s41467-017-00212-4.

Backbone

• Vector backbone: pLKO5d
• Total vector size (bp): 7408
• Modifications to backbone: modified pLKO.005
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dTomato
• gRNA/shRNA sequence: na
• Insert Size (bp): 4500

Gene/Insert 2

• Gene/Insert name: na
• gRNA/shRNA sequence: na

Gene/Insert 3

• Gene/Insert name: SFFV
• Alt name: Spleen Focus Forming Virus Promoter Enhancer
• gRNA/shRNA sequence: na
• Species: H. sapiens (human)
• Insert Size (bp): 450

Gene/Insert 4

• Gene/Insert name: miR30n
• Alt name: miR30
• gRNA/shRNA sequence: backbone
• Species: Synthetic
• Insert Size (bp): 450

Resource Information

• Supplemental Documents:
  • pLKO5d.SFFV.dTomato.miR30n.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry