pSAH031
(Plasmid
#90330)
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PurposePromoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonehigh-copy-number colE1/pMB1/pBR322/pUC origin of replication
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiLOV
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SpeciesA. thaliana (mustard weed), Synthetic
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Insert Size (bp)334
- Promoter None
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ACCTCTGACTTGAGCGTCG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSAH031 was a gift from David Savage (Addgene plasmid # 90330 ; http://n2t.net/addgene:90330 ; RRID:Addgene_90330)