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Addgene

pSH9
(Plasmid #90328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIM26
  • Backbone manufacturer
    William Mair
  • Backbone size w/o insert (bp) 4656
  • Total vector size (bp) 11180
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pges-1::set-2a
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    6524
  • Promoter ges-1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACTCCGAACTATGATGAC
  • 3′ sequencing primer TCAATTAAGATATCCACGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH9 was a gift from Anne Brunet (Addgene plasmid # 90328 ; http://n2t.net/addgene:90328 ; RRID:Addgene_90328)

  • For your References section:

    Mono-unsaturated fatty acids link H3K4me3 modifiers to C. elegans lifespan. Han S, Schroeder EA, Silva-Garcia CG, Hebestreit K, Mair WB, Brunet A. Nature. 2017 Apr 13;544(7649):185-190. doi: 10.1038/nature21686. Epub 2017 Apr 5. 10.1038/nature21686 PubMed 28379943
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