pmVirD2
(Plasmid
#90314)
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PurposemVirD2 expression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSNAP-tag® (T7)-2
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 6087
- Total vector size (bp) 6720
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Modifications to backboneinsertion of a T5 lac promoter upstream the gene
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameA. tumefaciens VirD2 from aa 1 to 204
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SpeciesA. tumefaciens
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Insert Size (bp)633
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GenBank IDM17989.1
- Promoter T7/T5 IPTG inducible
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Tag
/ Fusion Protein
- His6x tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer His tag universal primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmVirD2 was a gift from Bjorn Hogberg (Addgene plasmid # 90314 ; http://n2t.net/addgene:90314 ; RRID:Addgene_90314) -
For your References section:
Entirely enzymatic nanofabrication of DNA-protein conjugates. Bernardinelli G, Hogberg B. Nucleic Acids Res. 2017 Oct 13;45(18):e160. doi: 10.1093/nar/gkx707. 10.1093/nar/gkx707 PubMed 28977490