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Addgene

pRS313-Sad1-Wt
(Plasmid #90308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90308 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Backbone size w/o insert (bp) 4967
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SAD1
  • Alt name
    YFR005C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1346
  • Tag / Fusion Protein
    • no tag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SAD1 gene with 800 bp upstream of the start site and 900 bp downstream from the stop codon also included

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS313-Sad1-Wt was a gift from Christine Guthrie (Addgene plasmid # 90308 ; http://n2t.net/addgene:90308 ; RRID:Addgene_90308)

  • For your References section:

    The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. Hadjivassiliou H, Rosenberg OS, Guthrie C. RNA. 2014 May;20(5):656-69. doi: 10.1261/rna.042838.113. Epub 2014 Mar 28. 10.1261/rna.042838.113 PubMed 24681967
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