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pET28b-sad1-D159A
(Plasmid #90305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90305 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SAD1
  • Alt name
    YFR005C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1346
  • Mutation
    D159A
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-sad1-D159A was a gift from Christine Guthrie (Addgene plasmid # 90305 ; http://n2t.net/addgene:90305 ; RRID:Addgene_90305)

  • For your References section:

    The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. Hadjivassiliou H, Rosenberg OS, Guthrie C. RNA. 2014 May;20(5):656-69. doi: 10.1261/rna.042838.113. Epub 2014 Mar 28. 10.1261/rna.042838.113 PubMed 24681967
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