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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8
(Plasmid #90288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BB2_L_DE_syn_BbsI
  • Backbone size w/o insert (bp) 2215
  • Total vector size (bp) 7070
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pyrGtrunc
  • Species
    A .niger
  • Insert Size (bp)
    679
  • Mutation
    truncated version of pyrG gene (sequence is from 5' region)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CATTAATGCAGCTGGCAC
  • 3′ sequencing primer GGTTATTGTCTCATGAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8 was a gift from Michael Sauer (Addgene plasmid # 90288 ; http://n2t.net/addgene:90288 ; RRID:Addgene_90288)

  • For your References section:

    An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066
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