1121 pcDNA3 GFP FKHR H215R
(Plasmid
#9027)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 9027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6046
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKHR H215R
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Alt nameFKHR
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Alt nameFOXO1A
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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MutationH215R
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Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1121 pcDNA3 GFP FKHR H215R was a gift from William Sellers (Addgene plasmid # 9027 ; http://n2t.net/addgene:9027 ; RRID:Addgene_9027) -
For your References section:
Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Nakamura N, Ramaswamy S, Vazquez F, Signoretti S, Loda M, Sellers WR. Mol Cell Biol. 2000 Dec . 20(23):8969-82. 10.1128/MCB.20.23.8969-8982.2000 PubMed 11073996
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/54/22/2974ed54-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)