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Addgene

RFP-HA-DAT
(Plasmid #90265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    tagRFP-C1
  • Backbone size w/o insert (bp) 4725
  • Total vector size (bp) 6583
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DAT (dopamine transporter)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1863
  • Mutation
    inserted HA tag (see YFP-HA-DAT)
  • Entrez Gene
    SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer NO
  • 3′ sequencing primer NO
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RFP-HA-DAT was a gift from Alexander Sorkin (Addgene plasmid # 90265 ; http://n2t.net/addgene:90265 ; RRID:Addgene_90265)

  • For your References section:

    Dopamine transporter is enriched in filopodia and induces filopodia formation. Caltagarone J, Ma S, Sorkin A. Mol Cell Neurosci. 2015 Sep;68:120-30. doi: 10.1016/j.mcn.2015.04.005. Epub 2015 Apr 30. 10.1016/j.mcn.2015.04.005 PubMed 25936602
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