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pSL14 - Prp8 2142–2398
(Plasmid #90264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST15
  • Backbone manufacturer
    ThermoFisher Scientific
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRP8
  • Alt name
    RNA8
  • Alt name
    SLT21
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    only Jab1 domain (aa 2143-2398)
  • Entrez Gene
    PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal 6xHis tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ledoux and Guthrie, JBC, 2016

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL14 - Prp8 2142–2398 was a gift from Christine Guthrie (Addgene plasmid # 90264 ; http://n2t.net/addgene:90264 ; RRID:Addgene_90264)

  • For your References section:

    Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. Ledoux S, Guthrie C. J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 10.1074/jbc.M115.710848. Epub 2016 Apr 12. 10.1074/jbc.M115.710848 PubMed 27072132
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