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Addgene

pSL12 - Brr2 del422 Q904E
(Plasmid #90262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Backbone size w/o insert (bp) 4957
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRR2
  • Alt name
    RSS1
  • Alt name
    SLT22
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5226
  • Mutation
    deleted first 422 amino acids and amino acid Q904E
  • Tag / Fusion Protein
    • TAP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ledoux and Guthrie, JBC, 2017

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL12 - Brr2 del422 Q904E was a gift from Christine Guthrie (Addgene plasmid # 90262 ; http://n2t.net/addgene:90262 ; RRID:Addgene_90262)

  • For your References section:

    Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. Ledoux S, Guthrie C. J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 10.1074/jbc.M115.710848. Epub 2016 Apr 12. 10.1074/jbc.M115.710848 PubMed 27072132
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