pSL10 - Brr2 del422 WT
(Plasmid
#90260)
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Purposeprotein expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS313
- Backbone size w/o insert (bp) 4957
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBRR2
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Alt nameRSS1
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Alt nameSLT22
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)5226
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Mutationdeleted first 422 amino acids
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Tag
/ Fusion Protein
- TAP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ledoux and Guthrie, JBC, 2017
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL10 - Brr2 del422 WT was a gift from Christine Guthrie (Addgene plasmid # 90260 ; http://n2t.net/addgene:90260 ; RRID:Addgene_90260) -
For your References section:
Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. Ledoux S, Guthrie C. J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 10.1074/jbc.M115.710848. Epub 2016 Apr 12. 10.1074/jbc.M115.710848 PubMed 27072132
