1119 pBabe puroL HA FKHR AAA
(Plasmid #9025)

• Depositing Lab: William Sellers
• Publication: Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82.

Backbone

• Vector backbone: pBabe puro L
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKHR AAA
• Alt name: FKHR
• Alt name: FOXO1A
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: T24A S256A S319A
• Entrez Gene: FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBabe5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Oligonucleotides 5'-GCGCGGATCCATGGCCGAGGCGCCTCAGGTG-3' and 5'-CGCGCTCGAGGAATTCTCAGCCTGACACCCAGCTATG-3' were used to PCR amplify the FKHR cDNA. The PCR products, restricted with BamHI and XhoI, were ligated to similarly restricted pBABE-puroL to give pBABE-puroL-FKHR. AAA mutant cannot be phosphorylated by Akt.