YFP-HA-DAT R60A
(Plasmid
#90245)
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PurposeExpresses YFP-HA-DAT R60A mutant in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEYFP-C1
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 6589
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDAT (dopamine transporter)
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SpeciesH. sapiens (human)
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Insert Size (bp)1863
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Mutationinserted HA tag (see YFP-HA-DAT), mutated R60 to A
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Entrez GeneSLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NO
- 3′ sequencing primer NO (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-HA-DAT R60A was a gift from Alexander Sorkin (Addgene plasmid # 90245 ; http://n2t.net/addgene:90245 ; RRID:Addgene_90245) -
For your References section:
Negative regulation of dopamine transporter endocytosis by membrane-proximal N-terminal residues. Sorkina T, Richards TL, Rao A, Zahniser NR, Sorkin A. J Neurosci. 2009 Feb 4;29(5):1361-74. doi: 10.1523/JNEUROSCI.3250-08.2009. 10.1523/JNEUROSCI.3250-08.2009 PubMed 19193883
Map uploaded by the depositor.
