1099 pcDNA GFP FKHR AAA
(Plasmid #9023)

• Depositing Lab: William Sellers
• Publication: Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6046
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKHR AAA
• Alt name: FKHR
• Alt name: FOXO1A
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: T24A S256A S319A
• Entrez Gene: FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AAA mutant cannot be phosphorylated by Akt