YFP-DAT
(Plasmid #90228)

• Purpose: Expresses human DAT in mammalian cells
• Depositing Lab: Alexander Sorkin
• Publication: Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-83. Epub 2003 May 13.

Backbone

• Vector backbone: pEYFP-C1
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6589
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DAT (dopamine transporter)
• Species: H. sapiens (human)
• Insert Size (bp): 1863
• Entrez Gene: SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: NO
• 3′ sequencing primer: NO

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry