pMV762-Peredox-mCherry
(Plasmid #90217)

• Purpose: Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.
• Depositing Lab: Ashwani Kumar
• Publication: Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;6:145. eCollection 2016.

Backbone

• Vector backbone: pMV762
• Backbone size w/o insert (bp): 4563
• Total vector size (bp): 7326
• Vector type: Bacterial Expression ; Shuttle vector

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon Optimized Peredox-mCherry
• Species: Synthetic
• Insert Size (bp): 2763
• Promoter: groEL

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CGGCCAGCGTAAGTAGC
• 3′ sequencing primer: CCTGGCAGTCGATCGTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry