pSR83
(Plasmid #90211)

• Purpose: Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter
• Depositing Lab: Jeffrey Tabor
• Publication: Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. doi: 10.15252/msb.20167456.

Backbone

• Vector backbone: pSB4A3
• Backbone manufacturer: Registry of Standard Biological Parts
• Total vector size (bp): 5354
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: OmpR
• Species: E. coli
• Insert Size (bp): 720
• Entrez Gene: ompR (a.k.a. b3405, ECK3392, cry, kmt, ompB)

Gene/Insert 2

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711

Resource Information

• Supplemental Documents:
  • pSR83.str
• A portion of this plasmid was derived from a plasmid made by: Sebastian Schmidl, Tabor Lab, Rice University, Houston, TX, USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry