pSR78
(Plasmid #90210)

• Purpose: Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA
• Depositing Lab: Jeffrey Tabor
• Publication: Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. doi: 10.15252/msb.20167456.

Backbone

• Vector backbone: pACYCDuet-1
• Total vector size (bp): 8699
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CcaS
• Species: Synechocystis sp. PCC 6803
• Insert Size (bp): 2262

Gene/Insert 2

• Gene/Insert name: Cph8
• Species: Synechocystis sp. PCC 6803
• Insert Size (bp): 2235

Gene/Insert 3

• Gene/Insert name: Ho1
• Species: Synechocystis sp. PCC 6803
• Insert Size (bp): 723

Gene/Insert 4

• Gene/Insert name: PcyA
• Species: Synechocystis sp. PCC 6803
• Insert Size (bp): 747

Resource Information

• Supplemental Documents:
  • pSR78.str
• A portion of this plasmid was derived from a plasmid made by: Sebastian Schmidl, Tabor Lab, Rice University, Houston, TX, USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry