ALARM NMR
(Plasmid #90209)

• Purpose: Expresses the human La antigen for use in ALARM NMR
• Depositing Lab: Michael Walters
• Publication: Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi: 10.1038/s41467-017-01657-3.

Backbone

• Vector backbone: pET28b+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 6019
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: La antigen
• Alt name: SSB, La, L/Sjogren syndrome antigen B
• Species: H. sapiens (human)
• Insert Size (bp): 813
• Mutation: contains amino acids 100-324
• GenBank ID: NM_003142.4
• Entrez Gene: SSB (a.k.a. LARP3, La, La/SSB)
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • ALARMNMR-plasmid.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transform into Rosetta DE3 for protein expression