pIB190
(Plasmid #90197)

• Purpose: (Empty Backbone) E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter
• Depositing Lab: Indranil Biswas
• Publication: Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 10.1099/mic.0.2008/019265-0.

Backbone

• Vector backbone: pAMB1 (pORI23)
• Backbone manufacturer: Philippe Moreillon (PMID 10816506)
• Backbone size (bp): 6000
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • His-tag (N terminal on backbone)
  • Strep-tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • His-tag (N terminal on backbone)
  • Strep-tag (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • Biswas lab promoter/vector sequences
• A portion of this plasmid was derived from a plasmid made by: Contains MCS-B (from pASK43+). The backbone of this vector is based on pOri23- Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria.