pIB184-Km
(Plasmid #90195)

• Purpose: (Empty Backbone) E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter
• Depositing Lab: Indranil Biswas
• Publication: Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. doi: 10.1128/JB.00194-12. Epub 2012 Apr 13.

Backbone

• Vector backbone: pAMB1 (pORI23)
• Backbone manufacturer: Philippe Moreillon (PMID 10816506)
• Backbone size (bp): 6000
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • None (N terminal on backbone)
  • None (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • None (N terminal on backbone)
  • None (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • Biswas lab promoter/vector sequences
• A portion of this plasmid was derived from a plasmid made by: This plasmid is a theta vector containing the P23 promoter and MCS-A. The backbone of this vector is based on pOri23- Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a theta vector containing the P23 promoter and MCS-A. The backbone of this vector is based on pOri23- Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois).