pIB163
(Plasmid #90186)

• Purpose: (Empty Backbone) E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter
• Depositing Lab: Indranil Biswas
• Publication: Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 10.1099/mic.0.2008/019265-0.

Backbone

• Vector backbone: pSH71 (pJRS1315)
• Backbone manufacturer: June Scott (PMID 12867436)
• Backbone size (bp): 4000
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • His-tag (N terminal on backbone)
  • Strep-tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • His-tag (N terminal on backbone)
  • Strep-tag (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pIB163.gb
  • Biswas lab promoter/vector sequences
• A portion of this plasmid was derived from a plasmid made by: Contains MCS-B (from pASK43+). The backbone of this vector is based on pJRS1315-Dr. June Scott (Emory University) with additional components from pAL2-Dr. Vyv Salisbury (University of the West of England).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains MCS-B (from pASK43+). The backbone of this vector is based on pJRS1315-Dr. June Scott (Emory University) with additional components from pAL2-Dr. Vyv Salisbury (University of the West of England).

This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria.