pT2-cryR;dll4CE2-basEgfp
(Plasmid
#90144)
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Purposecontains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDESTtol2pACrymCherry
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Backbone manufacturerAddgene
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedll4E2basEgfp
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SpeciesD. rerio (zebrafish)
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Entrez Genedll4 (a.k.a. si:ch211-244p18.5, zgc:136596)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2-cryR;dll4CE2-basEgfp was a gift from Nathan Lawson (Addgene plasmid # 90144 ; http://n2t.net/addgene:90144 ; RRID:Addgene_90144) -
For your References section:
Robust Identification of Developmentally Active Endothelial Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq. Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND. Cell Rep. 2017 Jul 18;20(3):709-720. doi: 10.1016/j.celrep.2017.06.070. 10.1016/j.celrep.2017.06.070 PubMed 28723572