Flag-MCPIP1(delta ZF)
(Plasmid #90110)

• Purpose: Mammalian expression of Flag-MCPIP1(delta ZF)
• Depositing Lab: Mingui Fu
• Publication: Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. Epub 2010 Nov 29.

Backbone

• Vector backbone: pCMV-MAT-Tag-FLAG1
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4714
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCPIP1
• Alt name: Zc3h12a
• Alt name: regnase-1
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: K317G, S318G
• Entrez Gene: ZC3H12A (a.k.a. MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains K317G and S318G mutations in the ZF domain that render the domain nonfunctional. The rest of the ZF domain sequence is maintained.