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Addgene

Flag-MCPIP1(81-599)
(Plasmid #90107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-MAT-Tag-FLAG1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4714
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCPIP1
  • Alt name
    Zc3h12a
  • Alt name
    regnase-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    ZC3H12A (a.k.a. MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-MCPIP1(81-599) was a gift from Mingui Fu (Addgene plasmid # 90107 ; http://n2t.net/addgene:90107 ; RRID:Addgene_90107)

  • For your References section:

    MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-kappaB signaling. Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M. J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. Epub 2010 Nov 29. 10.1084/jem.20092641 PubMed 21115689
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