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Addgene

pMMB67EH-CFP
(Plasmid #90103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMB67EH
  • Backbone size w/o insert (bp) 9449
  • Total vector size (bp) 10154
  • Vector type
    Bacterial Expression ; Broad-host range

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CFP
  • Alt name
    CYPet
  • Alt name
    cyan fluorescent protein
  • Insert Size (bp)
    729
  • GenBank ID
    1253317
  • Promoter Ptac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer pBAD-Rvs
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMB67EH-CFP was a gift from Samuel Miller (Addgene plasmid # 90103 ; http://n2t.net/addgene:90103 ; RRID:Addgene_90103)

  • For your References section:

    Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Christen M, Kulasekara HD, Christen B, Kulasekara BR, Hoffman LR, Miller SI. Science. 2010 Jun 4;328(5983):1295-7. doi: 10.1126/science.1188658. 10.1126/science.1188658 PubMed 20522779
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