pMMB67EH-Spy
(Plasmid #90102)

• Purpose: IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.
• Depositing Lab: Samuel Miller
• Publication: Christen et al Science. 2010 Jun 4;328(5983):1295-7. doi: 10.1126/science.1188658.

Backbone

• Vector backbone: pMMB67EH
• Backbone size w/o insert (bp): 9449
• Total vector size (bp): 11618
• Vector type: Bacterial Expression ; Broad-host range

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YcgR
• Alt name: Spy
• Alt name: cyclic-di-GMP biosensor
• Alt name: YFP-YcgR-CFP
• Insert Size (bp): 2187
• GenBank ID: 1253317
• Entrez Gene: ycgR (a.k.a. b1194, ECK1182, JW1183)
• Promoter: Ptac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: pBAD-Rvs

Resource Information

• Supplemental Documents:
  • pMMB67EH-Spy.gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry