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Addgene

pNR-DEST
(Plasmid #90101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPtPBR1
  • Backbone size (bp) 10902
  • Modifications to backbone
    pPtPBR1 (episomal maintenance in the diatom P. tricornutum) was modified to include nitrate reductase (NR) promoter, (AttR1-CatR-ccdB-AttR2), and NR terminator.
  • Vector type
    Gateway Destination vector for protein expression in diatoms
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, Ampicillin, Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNR-DEST was a gift from Philip Weyman (Addgene plasmid # 90101 ; http://n2t.net/addgene:90101 ; RRID:Addgene_90101)

  • For your References section:

    Episomal tools for RNAi in the diatom Phaeodactylum tricornutum. Bielinski VA, Bolt TM, Dupont CL, Weyman PD. PeerJ Preprints. 2017 5:e2907v1 10.7287/peerj.preprints.2907v1
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