pH4-DEST
(Plasmid #90099)

• Purpose: (Empty Backbone) Gateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
• Depositing Lab: Philip Weyman
• Publication: Bielinski et al PeerJ Preprints. 2017 5:e2907v1

Backbone

• Vector backbone: pPtPBR1
• Backbone size (bp): 10591
• Modifications to backbone: pPtPBR1 (episomal maintenance in the diatom P. tricornutum) was modified to include H4 promoter, (AttR1-CatR-ccdB-AttR2), and FcpA terminator.
• Vector type: Gateway Destination vector for protein expression in diatoms
• Promoter: H4
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, Ampicillin, Tetracycline
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pH4-DEST.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry