pC013 - Twinstrep-SUMO-huLwCas13a
(Plasmid #90097)

• Purpose: Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.
• Depositing Lab: Feng Zhang
• Publication: Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.

Backbone

• Vector backbone: pET
• Backbone size w/o insert (bp): 6086
• Total vector size (bp): 9542
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Rosetta 2(DE3)pLysS Singles
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LwCas13a
• Alt name: LwC2c2
• Alt name: LwaCas13a
• Alt name: Cas13a/C2c2
• Species: Synthetic; Leptotrichia wadeii
• Insert Size (bp): 3280
• Tag / Fusion Protein:
  • 6xHis-Twin Strep-SUMO (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pc013-pet-his6-twinstrep-sumo-lwcas13a.gb
• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA - COVID 19 Research

Depositor Comments

Rosetta 2(DE3)pLysS Singles bacteria are BL21 derivative.This strain carries an additional chloramphenicol-resistant plasmid that supplies tRNAs for 7 rare codons to enhance expression of human codon optimized genes.