6His-MBP-TEV-huAsCpf1
(Plasmid #90095)

• Purpose: Bacterial expression plasmid for protein purification
• Depositing Lab: Feng Zhang
• Publication: Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.

Backbone

• Vector backbone: pET-28
• Backbone size w/o insert (bp): 6643
• Total vector size (bp): 10710
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21(DE3)pLysS
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: huAsCpf1
• Alt name: AsCas12a
• Species: humanized Acidaminococcus sp. BV3L6 Cpf1
• Insert Size (bp): 4056
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • MBP (N terminal on insert)
  • TEV site (N terminal on insert)
  • Nucleoplasmin NLS (C terminal on insert)
  • 3xHA (C terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: a Flag-AviTag containing fragment follows the insert (between the XhoI and HindIII sites) but this sequence is not translated.