1017 pcDNA3 Myr Akt1 deltaPH
(Plasmid #9009)

• Depositing Lab: William Sellers
• Publication: Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt1 dPH
• Alt name: Akt1
• Alt name: Akt
• Species: H. sapiens (human)
• Insert Size (bp): 1098
• Mutation: delta PH domain (aa1-130)
• GenBank ID: NP_005154
• Entrez Gene: AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
• Tag / Fusion Protein:
  • myr (MGSSKSKPKDPSQR) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry