pET21b-Caspase-3(C163S)
(Plasmid
#90088)
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PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 3
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SpeciesH. sapiens (human)
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MutationC163S
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
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Tag
/ Fusion Protein
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21b-Caspase-3(C163S) was a gift from Clay Clark (Addgene plasmid # 90088 ; http://n2t.net/addgene:90088 ; RRID:Addgene_90088) -
For your References section:
Removal of the pro-domain does not affect the conformation of the procaspase-3 dimer. Pop C, Chen YR, Smith B, Bose K, Bobay B, Tripathy A, Franzen S, Clark AC. Biochemistry. 2001 Nov 27;40(47):14224-35. bi011037e [pii] PubMed 11714276
