DD-Cas9 with filler sequence and Venus (EDCPV) Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid DD-Cas9 with filler sequence and Venus (EDCPV) with the following enzymes: StuI and EcoRV-HF.
We predict the following pattern:
StuI - 15.3 kb
EcoRV-HF - 15.3 kb
StuI+EcoRV-HF - 10.2 & 5 kb
We see the expected result.
