DD-Cas9 with filler sequence and Venus (EDCPV)
(Plasmid #90085)

• Purpose: This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
• Depositing Lab: Raffaella Sordella
• Publication: Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370.

Backbone

• Vector backbone: lentiCRISPR v2
• Backbone manufacturer: Addgene
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Species: Synthetic
• GenBank ID: 301447
• Promoter: EFS
• Tags / Fusion Proteins:
  • Destabilized Domain (N terminal on insert)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATTATCTAGAGCCACCATGGGAGTGCAGGTGGAAACCATCTCCCCAGGTGACG GGCGCACCTTCCCCAAGCGCG

Resource Information

• Supplemental Documents:
  • pLentiCRISPRv2.1_EFS-DD-Cas9-P2A-Ven (EDCPV).gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry