LLhE_AW-pHG165 H118R
(Plasmid #90063)

• Purpose: Constitutive expression of a chimeric LacI:CelR transcription repressor
• Depositing Lab: Liskin Swint-Kruse
• Publication: Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. doi: 10.1016/j.jmb.2015.09.015. Epub 2015 Sep 25.

Backbone

• Vector backbone: pHG165
• Backbone manufacturer: PMID: 3012611
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LLhE_AW/H118R
• Species: Synthetic
• Mutation: I48V/Q55A/Q60R/H118R
• Promoter: Iq

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli LacI DNA binding domain, LacI linker region, and T. fusca CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. CelR mutation is H121R but is referenced as H118R, which is a LacI/GalR family reference position number.