LPhP-pHG165
(Plasmid
#90047)
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PurposeConstitutive expression of a chimeric LacI:PurR transcription repressor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHG165
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Backbone manufacturerPMID: 3012611
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLPhP
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SpeciesSynthetic
- Promoter Iq
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E. coli LacI DNA binding domain, E. coli PurR linker region, and PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LPhP-pHG165 was a gift from Liskin Swint-Kruse (Addgene plasmid # 90047 ; http://n2t.net/addgene:90047 ; RRID:Addgene_90047) -
For your References section:
Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors: implications for sequence/function analyses. Tungtur S, Meinhardt S, Swint-Kruse L. J Mol Biol. 2010 Jan 29;395(4):785-802. doi: 10.1016/j.jmb.2009.10.001. Epub 2009 Oct 8. 10.1016/j.jmb.2009.10.001 PubMed 19818797