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Addgene

LLhS-pHG165a
(Plasmid #90040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHG165a
  • Backbone manufacturer
    PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LLhS
  • Species
    Synthetic
  • Promoter Iq

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E. coli LacI DNA binding domain, LacI linker region, and E. coli GalS regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. The GalS insert contains an E230K mutation compared to wild type.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLhS-pHG165a was a gift from Liskin Swint-Kruse (Addgene plasmid # 90040 ; http://n2t.net/addgene:90040 ; RRID:Addgene_90040)

  • For your References section:

    Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression. Meinhardt S, Manley MW Jr, Becker NA, Hessman JA, Maher LJ 3rd, Swint-Kruse L. Nucleic Acids Res. 2012 Nov;40(21):11139-54. doi: 10.1093/nar/gks806. Epub 2012 Sep 10. 10.1093/nar/gks806 PubMed 22965134
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