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pCS2TAL3_DD_NvFP-7R_Ex2-LEFT
(Plasmid #90036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    7R_Ex2-LEFT
  • Species
    Nematostella vectensis
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3x Flag (N terminal on insert)
    • Fok1-DD domain (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2TAL3_DD_NvFP-7R_Ex2-LEFT was a gift from Matthew Gibson (Addgene plasmid # 90036 ; http://n2t.net/addgene:90036 ; RRID:Addgene_90036)

  • For your References section:

    TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis. Ikmi A, McKinney SA, Delventhal KM, Gibson MC. Nat Commun. 2014 Nov 24;5:5486. doi: 10.1038/ncomms6486. 10.1038/ncomms6486 PubMed 25417547
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