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pEThT-RvLEAM
(Plasmid #90033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEThT
  • Backbone manufacturer
    Modified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RvLEAM
  • Alt name
    LC002821
  • Species
    Ramazzottius varieornatus
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert species is Ramazzottius varieornatus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033 ; http://n2t.net/addgene:90033 ; RRID:Addgene_90033)

  • For your References section:

    Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. Tanaka S, Tanaka J, Miwa Y, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Kubo T, Kunieda T. PLoS One. 2015 Feb 12;10(2):e0118272. eCollection 2015. DOI:10.1371/journal.pone.0118272 PubMed 25675104
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