1481 pcDNA3 flag HA 14-3-3 zeta
(Plasmid #9002)

• Depositing Lab: William Sellers
• Publication: 14-3-3 plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 14-3-3 zeta
• Alt name: 14-3-3
• Alt name: YWHAZ
• Species: B. taurus (bovine)
• Insert Size (bp): 750
• Mutation: T226A
• GenBank ID: NM_174814
• Entrez Gene: YWHAZ
• Tags / Fusion Proteins:
  • flag (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Created by Francisca Vazquez.

A T226A point mutation is found when Addgene's QC results are aligned with the noted Genbank sequence.